UTILIZATION OF ENVIRONMENTALLY FRIENDLY CARROT OIL AS A SUBSTITUTE FOR XYLOL IN THE DEPARAFINIZATION PROCESS

Main Article Content

indah sari

Abstract

Introduction: hematoxylin Eosin is a common dye used in histology laboratory examination. One of the stages of hematoxylin staining of eosin is the process of deparafinization. Deparafinisasi is a process to remove or dissolve paraffin from tissue and perform a clearing process before being observed under a microscope so that maximum color absorption at the time of tissue staining and look clear. The deparafinisai process is usually done by using xylol to dissolve paraffin in the form of fat. Xylol is commonly used as an ingredient for clearing and deparafinization processes. Continuous use of xylol can cause toxicity to the body and health. Medical laboratory workers, especially histology officers in charge of tissue coloring have a dangerous risk of chemical, biological, mechanical and environmental hazards so that a safer and cheaper alternative to xylol is needed. Alternative use of xylol is done to minimize harm to health and the environment, among others, by finding a substitute for xylol. Some natural ingredients such as carrot oil, pine oil, rose oil and olive oil can be used instead of xylol. Carrots relatively do not contain sulfur elements, so they do not cause air pollution, and can also improve the efficiency of agricultural resource utilization. Purpose of the study: to utilize environmentally friendly carrot oil as a substitute for xylol in the deparafinization process. Method: the research design used is true experiment design. Samples of human breast tissue (mammae) to see the results of the examination using an alternative reagent carrot oil and xylol as a control. The examination data will be processed by descriptive analysis based on the results of the assessment score of tissue preparation. Result: hematoxylin eosin staining using xylol get very good results of 100% while using carrot oil get very good results of 87.5% in the process of deparafinization. Discussion: Carrot oil can be used as a substitute for xylol in the process of deparafinization.

Article Details

How to Cite
1.
sari indah. UTILIZATION OF ENVIRONMENTALLY FRIENDLY CARROT OIL AS A SUBSTITUTE FOR XYLOL IN THE DEPARAFINIZATION PROCESS. maskermedika [Internet]. 30Dec.2024 [cited 19Feb.2025];12(2):364-70. Available from: https://jmm.ikestmp.ac.id/index.php/maskermedika/article/view/682
Section
Articles

References

Abdillah, F. S., & Sofyanita, E. N. (2023). Efektifitas Penggunaan Deterjen Laundry Cair sebagai Agen Deparafinisasi pada Sediaan Ginjal Mencit (Mus musculus). Borneo Journal of Medical Laboratory Technology, 5(2), 288–295. https://doi.org/10.33084/bjmlt.v5i2.5100
Akhtar, I., Javad, S., Jabeen, K., Saddiqe, Z., Ali Shah, A., & Aslam, F. (2023). A rapid recovery of phytochemicals from carrot seeds: an analytical approach. Journal of Taibah University for Science, 17(1). https://doi.org/10.1080/16583655.2023.2221051
Damayanti, M., Ariyadi, T., & TYAS, R. ayuning. (2022). Proses Deparafinasi Sediaan Jaringan Ginjal Dengan Dan Tanpa Pemanasan Menggunakan Mineral Oil Pada Pewarnaan Hematoksilin-Eosin. Jurnal Kesehatan Rajawali, 11(2), 1–6. https://doi.org/10.54350/jkr.v11i2.104
Deding, U., Baatrup, G., Kaalby, L., & Kobaek-Larsen, M. (2023). Carrot Intake and Risk of Developing Cancer: A Prospective Cohort Study. Nutrients, 15(3), 1–10. https://doi.org/10.3390/nu15030678
Haryo, A., Hartono, sugeng dwin, & Yuliana, R. (2021). Buku Dasar Forensik Veteriner (Cetakan Pe). Universitas Brawijaya Press.
Intan Sari, T., Hasanah, S., Arsyad, M., Ainur Rahmah, N., & Suciati, Y. (2022). Perbedaan Morfologi Kelenjar Mammae Dan Jaringan Ikat Pada Kanker Payudara Dan Fibrokistik Di Rumah Sakit Islam Jakarta. Majalah Sainstekes, 9(1), 001–008. https://doi.org/10.33476/ms.v9i1.2650
Mamay, M., Mutmaina, G. N., & Nurahma, I. A. (2022). Utilization Dishwashing Soap as a Subtitute of Xylol in the Deparaffinization process of Hematoxylin-Eosin Dye: Review Article. Medicra (Journal of Medical Laboratory Science/Technology), 5(1), 47–55. https://doi.org/10.21070/medicra.v5i1.1629
Mandrich, L., Esposito, A. V., Costa, S., & Caputo, E. (2023). Chemical Composition, Functional and Anticancer Properties of Carrot. Molecules, 28(20). https://doi.org/10.3390/molecules28207161
Mandrich, L., Piccolella, S., Esposito, A. V., Costa, S., Mercadante, V., Pacifico, S., & Caputo, E. (2023). Different Extraction Procedures Revealed the Anti-Proliferation Activity from Vegetable Semi-Purified Sources on Breast Cancer Cell Lines. Antioxidants, 12(6). https://doi.org/10.3390/antiox12061242
Margono, M. (2014). Ekstraksi Zat Warna Alami Wortel (Daucus Carota)Menggunakan Pelarut Air. Ekuilibium, 13(2), 51–54. https://doi.org/10.20961/ekuilibrium.v13i2.2160
Mayangsari, M. A., Nuroini, F., & Ariyadi, T. (2019). Perbedaan Kualitas Preparat Ginjal Marmut pada Proses Deparafinasi Menggunakan Xylol dan Minyak Zaitun pada Pewarnaan HE. Prosiding Mahasiswa Seminar Nasional Unimus, 2, 190–194.
Musnaini, M., Fransisca, S., & Leslie, W. (2022). Effectiveness Of Cream Formulation Of Carrot Seed Oil As Anti-Aging. International Journal of Health and Pharmaceutical (IJHP), 3(3), 331–340. https://doi.org/10.51601/ijhp.v3i3.170
Ramkita, N. (2022). Penanganan Jaringan Patologi Anatomi. Kementrian Kesehatan Direktorat Jendral Pelayanan Kesehatan.
Rehmat, S. (2023). A review on phytochemistry and risk reduction of cancers by Apium graveleons ( Celery ), Beta vulgaris ( Beet root ), Carota daucus ( Carrot ) and Citrus limon. August.
Rosnawaty, R., Suryati, Y., Sriati, A., Inayah, I., & Imelisa, R. (2022). Penurunan Beban Keluarga Dalam Merawat Klien Skizofrenia Melalui Triangle Terapy Di Masa Pandemik Covid-19. Jurnal Keperawatan Jiwa, 10(3), 535–544.
Saputra, R. (2016). Buku Ajar Statistik. In Forum Statistika dan Komputasi (Vol. 8, Issue 1).
Saraswati, & Rahmawati, Y. (2023). ROSELLA (Hibiscus sabdariffa) SEBAGAI ALTERNATIF BAHAN PEWARNA HISTOLOGI. JURNAL SAINS DAN TEKNOLOGI LABORATORIUM MEDIS, 9(1), 1–5.
Stanojević, J., Ilić, Z. S., Stanojević, L., Milenković, L., Kovač, R., Lalević, D., Šunić, L., Milenković, A., & Cvetković, D. (2023). Essential Oil Yield, Composition, and Antioxidant Activity in Two Umbel Maturity Stages of Wild Carrot (Daucus carota L. ssp. carota) from Montenegro. Horticulturae, 9(3). https://doi.org/10.3390/horticulturae9030328
Thomas, C., Erni, R., Wu, J. Y., Fischer, F., Lamers, G., Grigolon, G., Mitchell, S. J., Zarse, K., Carreira, E. M., & Ristow, M. (2023). A naturally occurring polyacetylene isolated from carrots promotes health and delays signatures of aging. Nature Communications, 14(1), 1–18. https://doi.org/10.1038/s41467-023-43672-7